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2 edition of Interspecific protoplast fusion in Streptomyces. found in the catalog.

Interspecific protoplast fusion in Streptomyces.

Paramaswari Somasundaram

Interspecific protoplast fusion in Streptomyces.

by Paramaswari Somasundaram

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Published by University of East Anglia in Norwich .
Written in English


Edition Notes

Thesis (M.Phil.) - University of East Anglia, School of Biological Sciences. 1983.

ID Numbers
Open LibraryOL13809282M

This chapter on Streptomyces focuses on the need to draw attention to the progress in developing systems for genetic manipulation in actinomycetes. A significant fraction of this progress has depended on approaches first used in Streptomyces species. Interestingly, the largest open reading frame in IS is related to that of members of the IS family.   Protoplast isolation and fusion 1. [email protected] 2. Somatic hybridization Somatic hybridiztion refers to crossing of crop plants though fusion of somatic cell. The fusion of somatic cell takes place through protoplasts. Protoplast is a plant, bacterial or fungal cell that had its cell wall completely or partially removed using either mechanical or enzymatic means.

In the context of plant genetic manipulation, somatic hybridization by protoplast fusion enables nuclear and cytoplasmic genomes to be combined, fully or partially, at the interspecific and intergeneric levels to circumvent naturally occurring sexual incompatibility barriers. Liposome-enhanced transformation of Streptococcus lactis and plasmid transfer by intergeneric protoplast fusion of Streptococcus lactis and Bacillus subtilis Jos M.B.M. Van der Vossen, Jan Kok, Daniel Van der Lelie and Gerard Venema Department of Genetics, University of Groningen, NN Haren, The Netherlands.

Fusion of protoplasts According to Kieser et al. (), protoplast fusion was performed between Streptomyces spp. CN as a donor strain and naturally chloramphenicol-resistant (camR) and S. coelicolor M as a recipient. 20 µg/ml chloramphenicol (fi n a l c oe t r) w su d recombinant strain. treatment(protoplast) {The two different strains after removal of cell wall forced to fuse using polyethylene glycol(PEG). {After the fusion, the cells are allowed to regenerate their cell wall. protoplast fusion technique for hybrid yeast Protoplast fusion and regeneration of a hybrid plant.


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Interspecific protoplast fusion in Streptomyces by Paramaswari Somasundaram Download PDF EPUB FB2

Rapid advances have been made in recent years on protoplast research in the economically important Streptomyces. The use of protoplasts has facilitated the development of efficient techniques for intra- and interspecific genetic recombination by fusion and by gene by: Progeny from a 4-factor interspecific protoplast-fusion cross between Streptomyces griseus and Streptomyces griseolus were analysed.

All 9 of the 14 possible progeny phenotypes detected by the selection procedure were identified and repeated progeny testing confirmed that the majority were genetically stable.

% of the cross progeny were by: 6. In general, interspecific protoplast fusion in the present study produced some new constructed isolates; Twenty out of 26 fusants lost their ability of inhibition zone formation with the tester.

Robinson M, Lewis E, Napier E () Occurrence of reiterated DNA sequences in strains of Streptomyces produced by an interspecific protoplast fusion. Mol Gen Genet – PubMed CrossRef Google ScholarCited by: 1. Intraspecific protoplast fusion was used to produce stable prototrophic recombinants of Streptomyces melanosporofaciens EF, a biocontrol agent of plant disease producing geldanamycin.

Intraspecific protoplast fusion was used to produce stable prototrophic recombinants of Streptomyces melanosporofaciens EF, a biocontrol agent. The recovery of protoplasts from conidia was % and the frequency of regeneration of protoplasts of 7'. oiride, T. koningii and T.

reesei strains was about 1% (data not shown), indicating the frequency of TABLE 2 FUSION FREQUENCIES IN THE VARIOUS COMBINATIONS OF AUXOTROPHIC MUTANTS OF TRICHODERMA SPECIES Parents No. of fusants/ Fusion frequency Strain A Strain B No.

of. Protoplast fusion has also been described for the production of improved yeast strains [72]. The technique of genome shuffling by protoplast fusion offers several advantages.

Protoplast fusion is a well-established technique that is applicable to an array of organisms including bacteria and both lower and higher eukaryotes. KEYWORDS Fusion; protoplast; regeneration; Bacillus INTRODUCTION Induced fusion of protoplasts by polyethylene glycol (PEG) was first demonstrated for plant cells (Kao and Michayluk, ) and has become a common technique for the investigation of genetics, differentiation and gene expression in fungi (Peberdy, ), yeast (van Solingen and van der Plaat, ), Streptomyces (Hopwood and.

Plant Protoplast Fusion and Growth of Intergeneric Hybrid Cells* Saskatoon, Saskatchewan S7N OW9, Canada Received J Summary. Interspecific and intergeneric fusions of plant protoplasts were induced by polyethylene glycol (PEG) or protoplast fusion, such as types of enzymes used for cell wall digestion, periods of.

Interspecific electrofusion between protoplasts of multhiomycin-producing Streptomyces antibioticus aAL Ala − Leu − and neomycin-producing S. fradiae fHL His − Leu − was done. When the concentration of both protoplasts increased to 1 × 10 9 protoplasts/ml, the frequency of fusion.

Protoplast fusion was shown to produce high frequencies of recombinant progeny in intraspecies crosses with auxotrophic mutants of Streptomyces canescens, Streptomyces griseus and Streptomyces.

Protoplast fusion is a physical phenomenon,during fusion two or more protoplasts come in contact and adhere with one another either spontaneously or in presence of fusion inducing agents.

ABSTRACT: Protoplast fusion between different species of Streptomyces was performed using a liquid regeneration method developed for a rapid and simple preparation of the fusants.

Consequently, new clones, which could not be obtained using the conventional agar regeneration method, were obtained. In the crosses between S. griseus and S.

durhamensis, and. Summary DNA isolated from a Streptomyces strain produced by interspecific protoplast fusion of Streptomyces jumonjinensis and Streptomyces lipmanii contained a tandemly repeated six kilobase pair sequence which constituted 15%–18% of the total DNA.

S.S. et al ).Protoplast fusion may be used to produce interspecific or even intergeneric last fusion becomes an important tool of gene manipulation because it breakdown the barriers to genetic exchange imposed by conventional mating last fusion technique has a great potential for genetic analysis and for strain.

Genetic Interactions in Streptomyces rimosus Mediated by Conjugation and by Protoplast Fusion Article (PDF Available) in Journal of general microbiology (5) June with 34 Reads. A procedure for efficient transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts with plasmid DNA was developed.

Transformation frequencies with S. fradiae protoplasts were strongly influenced by the temperatures for cell growth, protoplast formation, and protoplast regeneration. Transformation frequencies for both species were also influenced by the culture age.

Here we first improved the ε-PL productivity in five species of wild-type strains in Streptomyces (Streptomyces padanus, Streptomyces griseofuscus, Streptomyces graminearus, Streptomyces hygroscopicus, and Streptomyces albulus) by genome shuffling.

Then all the shuffled strains were suffered from an interspecific hybridization through stochastic protoplast fusion. The frequency of recombination between Streptomyces hygroscopicus var. yingchengensis and Streptomyces qingfengmyceticus as a result of interspecific protoplast fusion was x 10(-4).

Characterization of biological properties revealed differences in spore mass color, drug resistance, antimicrobial activity, and antibiotic production capacity. Interspecific protoplast fusion among macrolide-producing streptomycetes.

Ikeda H, Inoue M, Tanaka H, Omura S. From auxotrophic and idiotrophic mutants of Streptomyces fradiae (tylosin producer) and Streptomyces sp. AM (pikromycin producer) or Streptomyces narbonensis (narbomycin producer), prototrophic fusants were obtained at a low.PDF ( K) PDF-Plus ( K) Citing articles; Interspecies matings of Streptomyces fradiae with Streptomyces bikiniensis mediated by conventional and protoplast fusion techniques.

O. Godfrey, L. Ford, and, M. L. B. Huber.Breeding of edible mushrooms using the technique of spore-crossing is only applicable to intraspecific hybridization in monokaryotic strains. Thus, an alternative approach to the breeding of basidiomycetes involving protoplast fusion is now being widely adopted in order to obtain interspecific and intergeneric hybrids which produce fruit bodies of desirable quality.